C4Legio LFA

Legionnaires’ disease

Legionella Pneumophila in lugs, 3D

Human lungs infected by Legionella pneumophila

What’s Legionnaires’ disease ?

Legionnaires’ disease is a severe pneumonia caused by bacteria of the genus Legionella. One usually gets it by breathing in mist from water that contains the bacteria. The mist may come from hot tubs, showers or air-conditioning units for large buildings.

Symptoms of Legionnaires’ disease include high fever, chills, a cough and sometimes muscle aches and headaches. But other types of pneumonia have similar symptoms. So, lab tests are required to detect the specific bacteria that causes Legionnaires’ disease.

Key figures about Legionellosis

Up to

%

mortality rate (1)

From 20 to

%

of cases stay non-diagnosed (3)

More than

cases in Europe in 2019 (1)

10 000 in the US in 2018 (2)

Increase of

%

of cases' number in Europe from 2017 to 2019 (1)

The standard of care today

Currently, there are two types of tests for Legionnaires’ disease diagnosis :

Culture-based tests (gold standard)
This test has a 5 to 10 days turn-around-time (TAT), detects all serogroups, but is operator and lab-dependent. As a results, sensitivity ranges from 10 to 80%.

Urinary antigens tests

This test has a short TAT (20 minutes), but detects reliably only serogroup 1 (there are 15 serogroups of Legionella). Serogroup 1 is the most common (50 to 80% of cases), but all are deadly.

Combining rapid and accurate diagnosis is game-changing: mortality rate is < 10% for patients treated within 48h, while it ramps up to almost 50% for patients treated 48 hours after the first signs of infection.

Patient Care, a priority at C4Diagnostics
Patient Care, a priority at C4Diagnostics

The main features of C4Legio LFA

C4Legio LFA™ is a qualitative detection test of all Legionella pneumophila (Lp) serogroups in human bronchoalveolar lavages (BAL) and tracheal aspirates.

This test developed by C4Diagnostics to detect Legionnaires’ disease answers the above-mentioned limitations and enables :

  • detection of all serogroups of Legionella
  • turn-around time of 48 hours

It is intended to be used by professional users, such as laboratory technicians and biologists.

The first version of the test was a Research Use Only version (C4Legio). In 2021, we have moved to a second version using the lateral flow assay (C4Legio LFA).

The CE marking is ongoing.

Key features of C4Legio LFA

Lateral flow assay (LFA)

All Lp serogroups detected

Detection in broncho-alveolar lavages and tracheal aspirates

Based on Click chemistry technology

Results in 48 hours

Compact reader connected to LIMS

Relevant publications